Composite

Part:BBa_K3610044:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-10-10)


EFR ectodomain / SmallBit NanoLuc for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 93
    Illegal NheI site found at 235
    Illegal NheI site found at 1165
    Illegal NheI site found at 2083
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 148
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1751
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization was done with SnapGene

Source

BBa_K2136017, BBa_K3610012, BBa_K3610008, BBa_M10019, BBa_K416001, BBa_K3610017

References

Kunze, Gernot; Zipfel, Cyril; Robatzek, Silke; Niehaus, Karsten; Boller, Thomas; Felix, Georg (2004): The N terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants. In: Plant Cell 16 (12), S. 3496–3507. DOI: 10.1105/tpc.104.026765.

England, Christopher G.; Ehlerding, Emily B.; Cai, Weibo (2016): NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. In: Bioconjug Chem 27 (5), S. 1175–1187. DOI: 10.1021/acs.bioconjchem.6b00112.

Barahimipour, Rouhollah; Strenkert, Daniela; Neupert, Juliane; Schroda, Michael; Merchant, Sabeeha S.; Bock, Ralph (2015): Dissecting the contributions of GC content and codon usage to gene expression in the model alga Chlamydomonas reinhardtii. In: Plant J 84 (4), S. 704–717. DOI: 10.1111/tpj.13033.